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Figure 2. Establishment of the HSC Program and Arterial Specification of the DA Is Abolished in Tel1-Depleted Embryos
(A) In situ hybridization analysis showing that expression of hematopoietic genes in the ventral wall of the DA (arrows) is absent in Tel1 morphants. In contrast, expression in embryonic erythrocytes (Runx1, Scl, and Lmo2, arrowheads) and myeloid cells (SpiB and Gfi1, arrowheads) is still observed.
(B) Expression analysis showing that the DA (arrows) of Tel1 morphants does not express the arterial affiliated genes, Notch4, Dll4, and EphrinB2, nor the Notch target, Gata2.
(C) Endothelial expression of Erg1 reveals the presence of DA precursors (arrows) coalescing in the midline of Tel1 morphants. All embryos were hybridized as whole mounts and are shown in lateral view, with anterior to the left and dorsal to the top. Stages of development are as indicated. The numbers of embryos represented by each panel, out of the number analyzed, are indicated in the top right corner. See also Figure S1 and Table S1.
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Figure 1. Tel1 Is Expressed in the Embryonic DA and Is Required for the Emergence of HSCs
(A) In situ hybridization on transverse sections showing expression of Tel1 in the ventral wall of the DA and emerging HSCs (red arrows).
(B) Scl staining on transverse sections shows that intra-aortic hematopoietic clusters containing HSCs do not emerge in Tel1 morphants. All sections shown are at 40× magnification, with dorsal to the top. g, ganglia; n, notochord; PCV, posterior cardinal vein; Pd, pronephric duct. Stages of development are as indicated. See also Figures S1 and S2, and Table S1. |
