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Fig. 5. SCL expression in Xvent-1 RNA, Xvent-2 RNA or pCSKA-Xwnt-8-injected embryos. Fertilized eggs were microinjected with 1.0 ng of
GFP RNA, 1.0 ng of Xvent-1 RNA, 4.0 ng of Xvent-2 RNA or 100 pg of pCSKA-Xwnt-8, and the injected embryos were assayed for SCL
expression at stage 24 by (A) in situ hybridization, and (B) northern analysis. (A) Xvent-1-injected embryos showed the greatest degree of
variation and examples of elevated (left), control-like (center) and reduced (right) levels of expression are shown. (C) For Xvent-1 injection,
optical densities of bands from northern blots were calculated using NIH image software, and the values are shown for four independent
experiments after adjusting for total RNA loading by EF1a hybridization intensity. |