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Figure S5. Down regulation of hemangioblast markers occurs before apoptosis in
the primitive and definitive hemangioblast populations.
(A-D) TUNEL assay using in situ cell death assay kit. At st14, Scl expression is
downregulated (A,B red circles) but there is no apoptosis, whereas at st17, apoptosis is
clear (D, white dots within white circle) and little Scl expression remains (D, black
arrows) compared with controls (C, red circle). (G-J) TUNEL assay using Apoptag kit.
At st24, although Scl expression is absent in Fli1 morphants (E,F) [apparent staining just
above the black arrow is trapping], no apoptosis is seen in the DLP (G,H, arrows), but
some is seen in the somites of both control and morphant embryos. At st26, TUNEL
signals are detected in Fli1 morphants (J, arrow) which includes the DLP and PND.
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