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Fig. 3. Co-injection of tsg mRNA rescues the tsg morphant phenotype. (A)NOT SHOWN: Alignment of the target sequence for the tsg MO used in this study with the 5V region of mRNAs for X. tropicalis, X. laevis and Oncorhynchus mykiss (rainbow trout). The translational start site is shown in green, mismatches between O. mykiss and the tsg MO are shown in pink, and mismatches between X. laevis and the tsg MO are shown in blue. (B) NOT SHOWN: Embryos were injected at the two-cell stage with 20 ng tsg MO or 20 ng tsg MO plus 400 pg of O. mykiss tsg mRNA, then assayed at stage 26 for survival and proper blastopore closure. The experiment was repeated with the same result. (C) Embryos were injected with 400 pg O. mykiss tsg mRNA (b, f, j, n, r, v), 20 ng tsg MO (c, g, k, o, s, w), or both (d, h, l, p, t, x) and compared to uninjected sibling control embryos (a, e, i, m, q, u) at stage 11 (a l), stage 19 (m t), or stage 26 (u x) for expression of the molecular markers indicated at left. The view for each marker is indicated at right. For animal and vegetal views, dorsal is to the top while for ventral and lateral views, anterior is to the left. |
