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Fig. 2. Tsg morphants are ventralized. (A) Embryos were injected at the two-cell stage with a high dose of tsg MO (g l) and compared to uninjected sibling control embryos (a) at stage 11 for changes in morphology and molecular marker expression as indicated. Arrows in panels e, k indicate the dorsal limit of sizzled expression, while arrows in panels f, l indicate the animal limit of vent-2 expression. Embryos in panels f, l are shown from a dorsal view, all others are vegetal views with dorsal to the top. (B) Embryos injected with low (b, f, j, n, r), intermediate (c, g, k, o, s) or high (d, h, l, p, t) doses of tsg MO were compared to uninjected control siblings (a, e, i, m, q) for changes in morphology and expression of the ventral molecular marker sizzled (e, m) and the dorsal forebrain marker eomesodermin (q t). All embryos are shown in lateral views with dorsal to the top, anterior to the left. Embryos were analyzed at stage 15 (a h) or stage 25 (i t). The arrowhead in panel l marks the cement gland. |