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Experiment details for spry4

Wang YH and Beck CW (2014) Assay

Distal expression of sprouty (spry) genes during Xenopus laevis limb development and regeneration.

Gene Clone Species Stages Anatomy
spry4.L laevis NF stage 32 olfactory placode , otic vesicle , pharyngeal arch , mandibular arch , branchial arch , [+]

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  Fig. 1. Identification of three spry genes in a stage 51 limb bud transcriptome. (A) stacked column graph showing percentage of reads originating from each third of the limb bud: proximal, middle or distal. fgf8 and -8b are included for comparison. (B) Bar graph to show relative numbers of reads for each transcript across the whole limb, normalised for transcript size. Higher numbers of reads indicate higher levels of expression. (C–F) In situ hybridisation to stage 32 X. laevis embryos for spry1a, 2, 3 and 4. Abbreviations: ba: branchial arches, mhb: mid/hindbrain boundary, op: olfactory placode, ov: otic vesicle, sc: spinal cord, tb: tail bud.

Gene Clone Species Stages Anatomy
spry4.L laevis NF stage 32 olfactory placode , otic vesicle , pharyngeal arch , branchial arch , tail bud

  spry4 (sprouty homolog 4) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 32, lateral view, anterior left, dorsal up.

Gene Clone Species Stages Anatomy
spry4.L laevis NF stage 50 to NF stage 51 hindlimb bud , distal mesenchyme
spry4.L laevis NF stage 52 blood vessel , hindlimb bud , distal mesenchyme
spry4.L laevis NF stage 53 blood vessel , hindlimb bud , distal mesenchyme , autopod , hindlimb stylopod , [+]
spry4.L laevis NF stage 54 hindlimb bud , distal mesenchyme , hindlimb digit 3 , hindlimb digit 4
spry4.L laevis NF stage 55 blood vessel , hindlimb bud , distal mesenchyme , autopod , hindlimb digit 3 , [+]

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  Fig. 2. In situ hybridisation of spry1a, spry2, spry4 and fgf8b in Xenopus laevis developing hindlimbs from stage 49 to 55. Fgf8b is included to show the location of the AER at developmental stages 50–54. Specific staining is dark purple. Black spots in some panels are melanophores in pigmented specimens. Black arrows indicate distal expression, red arrows localised expression in more proximal mesenchyme of older limbs, and thin white arrows indicate possible forming blood vessels. Roman numerals show detectable staining in some digits. The AER is indicated in some panels to show absence of spry1a and spry4 and presence of spry2 and fgf8b transcripts. All limbs are oriented proximal to the left and posterior uppermost, with the tadpole on its right side, except for panels B, I and V where both hind limbs are viewed from the anterior with the tadpole placed on its back. For stage 54 and 55 limbs only the autopod is shown for spry1a, spry4 and fgf8b, as by this stage there is no proximal staining. Developmental stage is in the bottom right of each panel and limbs are photographed to the same scale, except for panel T which shows histological section through a stage 53 limb bud to show expression of spry2 in the AER around digit IV. Scale bar in T is 50 um.

Gene Clone Species Stages Anatomy
spry4.L laevis NF stage 50 hindlimb bud , distal mesenchyme , hindlimb bud tip

  spry4 (sprouty homolog 4) gene expression in Xenopus laevis embryonic hindlimb, assayed via in situ hybridization, NF stage 50, proximal to the left and posterior uppermost, with the tadpole on right.

Gene Clone Species Stages Anatomy
spry4.L laevis NF stage 51 hindlimb bud , distal mesenchyme

  spry4 (sprouty homolog 4) gene expression in Xenopus laevis embryonic hindlimb, assayed via in situ hybridization, NF stage 51, proximal to the left and posterior uppermost, with the tadpole on right.

Gene Clone Species Stages Anatomy
spry4.L laevis NF stage 52 blood vessel , hindlimb bud , distal mesenchyme

  spry4 (sprouty homolog 4) gene expression in Xenopus laevis embryonic hindlimb, assayed via in situ hybridization, NF stage 52, proximal to the left and posterior uppermost, with the tadpole on right.

Gene Clone Species Stages Anatomy
spry4.L laevis NF stage 53 blood vessel , hindlimb bud , distal mesenchyme , autopod , hindlimb stylopod , [+]

  spry4 (sprouty homolog 4) gene expression in Xenopus laevis embryonic hindlimb, assayed via in situ hybridization, NF stage 53, proximal to the left and posterior uppermost, with the tadpole on right.

Gene Clone Species Stages Anatomy
spry4.L laevis NF stage 54 blood vessel , hindlimb bud , distal mesenchyme , hindlimb digit 3 , hindlimb digit 4

  spry4 (sprouty homolog 4) gene expression in Xenopus laevis embryonic hindlimb, assayed via in situ hybridization, NF stage 54, proximal to the left and posterior uppermost, with the tadpole on right.

Gene Clone Species Stages Anatomy
spry4.L laevis NF stage 55 blood vessel , hindlimb bud , distal mesenchyme , autopod , hindlimb digit 3 , [+]

  spry4 (sprouty homolog 4) gene expression in Xenopus laevis embryonic hindlimb, assayed via in situ hybridization, NF stage 55, proximal to the left and posterior uppermost, with the tadpole on right.