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sox9xenopus   

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Experiment details for sox9

O'Donnell M et al. (2006) Assay

Functional analysis of Sox8 during neural crest development in Xenopus.

Gene Clone Species Stages Anatomy
sox9.S laevis NF stage 12.5 neural crest , neural plate border
sox9.S laevis NF stage 14 neural crest , neural plate border

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  Fig. 4. Sox8 regulates the onset of expression of Slug and Sox9. (A) Embryos injected in one blastomere at the two-cell stage with 30 ng of Sox8mo exhibited reduced Sox9 and Slug expression at stage 12.5 and stage 14. Lateral view in all panels, anterior is towards the right (control side) or to the left (injected side). (B) Quantification of the in situ hybridization results. The numbers in parentheses indicate the number of embryos analyzed.

Gene Clone Species Stages Anatomy
sox9.S laevis NF stage 14 to NF stage 15 neuroectoderm , neural crest
sox9.S laevis NF stage 25 mandibular arch , hyoid arch , branchial arch , otic placode , optic vesicle , [+]
sox9.S laevis NF stage 35 and 36 otic vesicle , brain , forebrain , midbrain , neural crest , [+]

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  Fig. 1. Sox8 expression in neural crest progenitors and their derivatives. (A) Sox8 is first detected at the gastrula stage in a ventrolateral domain around the blastopore. At stage 11.5 (B-D) Sox8 expression around the blastopore persists and additional expression is detected lateral to the neural plate (arrows). Vegetal (A), lateral (B,C; anterior towards right) and dorsal (D; anterior towards the top) views. Comparison of Sox8 (E) Sox9 (F) and Sox10 (G) expression in sibling stage 14/15 embryos illustrates that all three genes are expressed in the presumptive neural crest. Dorsal views, anterior towards the top. (H) Section of a stage 15 embryo illustrates the expression of Sox8 in both the lateral (arrows) and the medial neural crest (arrowheads). At stage 17 (I,J), Sox8 persists in the neural crest region and is also expressed anterior to the neural plate in the prospective cement gland (arrow). As the neural tube closes (K,L), Sox8 is detected in the migrating neural crest cells in the cranial region (arrows), and the premigratory cells in the trunk neural crest (arrowheads). Dorsal view, anterior towards top (I,K); lateral view, anterior towards the left (L); cranial view (J). (M) Comparison of Sox8, Sox9 and Sox10 expression at the tailbud stages. Dorsal views of stage 25 embryos, anterior towards the right. Sox8, Sox9 and Sox10 are co-expressed in the migrating cranial neural crest. Posteriorly, although Sox8 and Sox10 are both expressed in trunk neural crest cells, Sox9 is downregulated in this cell population. At stage 35 (left panels, lateral views), Sox8 is detected in the cranial neural crest similar to Sox9; however, at this stage, Sox10 starts to be downregulated in the branchial arches. Sox8, Sox9 and Sox10 are co-expressed in the otic vesicle at this stage (arrows). Ventral views (right panels) showing colocalization of Sox8 and Sox9 in the pancreatic rudiments (arrowheads) where Sox10 is not detected.

Gene Clone Species Stages Anatomy
sox9.S laevis NF stage 17 neural crest , cranial neural crest

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  Fig. 3. Sox8-depleted embryos fail to express Sox10 at the neural plate border. (A) Increasing amounts of Sox8mo (10 ng, 100 ng and 1000 ng) blocks translation directed by Sox8 mRNA. The same morpholino (500 ng) fails to block Sox9 and Sox10 translation. (B) Embryos injected in one blastomere at the two-cell stage with 30 ng of Sox8mo exhibit reduced Sox10 expression at stage 17, while Slug, Sox9 and Sox2 expression appears largely unaffected at this stage. (C) Quantification of the in situ hybridization results. The numbers in parenthesis indicate the number of embryos analyzed. (D) Sox8mo (500 ng) does not interfere with translation of a mutated Sox8 mRNA (mSox8). (E) Rescue experiments were performed by injection of an animal dorsal blastomere at the eight-cell stage. Bilateral Sox10 expression is rescued in Sox8mo-injected embryo by co-injection of mSox8 mRNA (Sox8mo+mSox8). Single injection of mSox8 expanded the Sox10 expression domain. Injection of a 5 bp mis-matched Sox8 morpholino (Sox8mis) had no effect on Sox10 expression. (F) Quantification of the in situ hybridization results. The numbers in parentheses indicate the number of embryos analyzed. (B,E) Dorsal view, anterior is towards the top. RNA encoding the lineage tracer β-galactosidase was co-injected to identify the injected side (red staining) (left side in B and right side in E).