| |
Fig. S6. Glycolysis and PI3K-Akt signaling control neural crest migration. Glycolysis blockade (AJ)
and PI3K-Akt blockade (K-T) during EMT and neural crest migration (stage 18 to stage 24) affects
neural crest migration as shown by the expression of Sox9, Sox10 and Twist. Embryos rinsed at stage
24 and grown until stage 45 display craniofacial and eye development defects, albeit the presence of
all individual cartilage elements. (A-C,F-H,K-M,P-R, U-W) Side views, anterior is to the left. (D,I,N,S,X)
dorsal views st 45. (E,J,O,T,Y) Ventral views of alcian blue stained visceral cartilages, st 45. Scale bar=
500μm. |
