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Fig. 4. Snail2 requires EZH2 to regulate neural crest markers. Snail2 RNA (0.15 ng) was injected alone or with 20 ng EZH2-MO unilaterally into two-cell-stage embryos. ISH performed at neurula stages 17-18 revealed that ectopic expression of Snail2 expanded domains of neural crest genes Snail2, Sox9, Sox10 and Twist. However, Snail2 failed to expand the domains of these genes in the presence of EZH2-MO, implying that neural crest expansion by Snail2 required EZH2 function. The numbers of embryos showing the displayed patterns over total embryos analyzed are shown. |
