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δXLef1 and δXTcf3 differ in their ability to influence neural crest induction. (A) Embryos injected with mRNA encoding β-gal and δXLef1 nMT (i, iii, v) or δXTcf3 nMT (ii, iv, vi) were examined by in situ hybridization for expression of Slug (i, ii); Sox9 (iii, iv); and Msx1 (v, vi), with .denoting probe used. All embryos are positioned showing anterior/dorsal side, with their injected side to the right (arrow). δXTcf3 potently blocks expression of neural crest markers while δXLef1 does not. (B) Schematic representation of constructs utilized in these experiments compared to full-length coding regions. (C) Western blot demonstrating that δXLef1 and δXTcf3 were expressed at equivalent levels in these experiments; blot was stripped and re-probed for actin as a loading control. (D) Expression of either δXLef1 nMT or δXTcf3 nMT leads to an increase in Opl/Zic1 expression. Light blue staining is lineage tracer β-gal. |
