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Fig. S10. Knockdown of Ripply3 disrupts placode morphology in the tailbud stage. All embryos were unilaterally injected at the two- or four- cell stage with 50 ng Ripply3 MO 1+2. Lateral views of the same embryo are shown facing each other. (A,C,E) Uninjected side showing control expression of Eya1, Six1 and Sox9. (B,D,F) Injected side showing altered placode morphology in Eya1 and Six1 in the spacing of the epibranchial placodes (EB), blurring of the lateral line placodes (LL), otic placode (Ot) and trigeminal placode (TG). The spacing of the hyoid crest (HC) and branchial crest (BC) is also altered in Sox9 expression. MC, mandibular crest; L, lens. |
