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Experiment details for sox9

Differential distribution of competence for panplacodal and neural crest induction to non-neural and neural ectoderm.

Differential distribution of competence for panplacodal and neural crest induction to non-neural and neural ectoderm.

Gene Clone Species Stages Anatomy
sox9.S laevis NF stage 18 neural crest , trunk neural crest , cranial neural crest , anterior placodal area

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  Fig. 4. Effects of Dlx3 or GATA2 gain or loss of function on neural ectodermal markers. (A-G′′) Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows), and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Double asterisks indicate lateral displacement owing to widening of the neural plate. For Msx1, additional examples of embryos are shown in the insets in C and C′.

Gene Clone Species Stages Anatomy
sox9.S laevis NF stage 18 epibranchial placode , cranial neural crest , posterior placodal area

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  Fig. S3. Effects of Dlx3 or GATA2 loss of function after injection of engrailed repressor constructs on neural and non-neural ectodermal markers. Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows), and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Double asterisks indicate lateral displacement owing to widening of the neural plate. See text for details.

Gene Clone Species Stages Anatomy
sox9.S laevis NF stage 22 mandibular crest , hyoid crest , branchial crest , cranial neural crest

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  Fig. 1 . Induction of ectodermal markers at neural plate boundaries. Ectodermal marker expression in tailbud stage embryos (albino) that had received neural plate grafts (pigmented and/or mycGFP-labeled) into belly ectoderm at stage 13. (A-F′′) Grafts (arrows, outlined) are shown in overviews (A-F) and transverse sections in brightfield (A′-F′), green fluorescent channel (A′-F′), and overlay (A′′-F′′). Sox3 (A-A′′), Sox11 (B-B′′), Zic1 (C-C′′; inset shows higher magnification of expression in graft) and FoxD3 (D-D′′) are maintained or induced (asterisks) in graft. Six1 (F-F′′) is induced in host ectoderm (arrowheads), whereas Sox9 (E-E′′) is induced in the graft (asterisk) and sometimes in host ectoderm (arrowhead).