|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
zic1
|
|
laevis
|
NF stage 18
|
cranial neural crest
,
neural plate border
|
pax3
|
|
laevis
|
NF stage 18
|
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
,
anterior placodal area
|
sox3
|
|
laevis
|
NF stage 20
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
anterior neural fold
,
anterior placodal area
|
foxd3
|
|
laevis
|
NF stage 18
|
cranial neural crest
|
msx1
|
|
laevis
|
NF stage 18
|
neural plate border
,
anterior placodal area
|
tubb2b
|
|
laevis
|
NF stage 18
|
Rohon-Beard neuron
,
neuron
,
anterior placodal area
|
|
|
Fig. 4. Effects of Dlx3 or GATA2 gain or loss of function on neural ectodermal markers. (A-G′′) Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows), and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Double asterisks indicate lateral displacement owing to widening of the neural plate. For Msx1, additional examples of embryos are shown in the insets in C and C′. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gata2
|
|
laevis
|
NF stage 18
|
non-neural ectoderm
|
eya1
|
|
laevis
|
NF stage 18
|
anterior placodal area
,
posterior placodal area
|
six1
|
|
laevis
|
NF stage 18
|
anterior placodal area
,
posterior placodal area
|
zic1
|
|
laevis
|
NF stage 18
|
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
,
posterior placodal area
|
pax3
|
|
laevis
|
NF stage 18
|
chordal neural plate
,
profundal placode
,
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
|
sox3
|
|
laevis
|
NF stage 18
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
neural plate border
,
anterior placodal area
|
foxd3
|
|
laevis
|
NF stage 18
|
cranial neural crest
|
msx1
|
|
laevis
|
NF stage 18
|
neural plate
,
presumptive midbrain-hindbrain boundary
,
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
,
[+]
|
dlx5
|
|
laevis
|
NF stage 18
|
non-neural ectoderm
,
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
|
foxi2
|
|
laevis
|
NF stage 18
|
anterior placodal area
|
dlx3
|
|
laevis
|
NF stage 18
|
non-neural ectoderm
,
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
|
krt12.4
|
|
laevis
|
NF stage 18
|
epidermis
,
non-neural ectoderm
|
|
|
Fig. S3. Effects of Dlx3 or GATA2 loss of function after injection of engrailed repressor constructs on neural and non-neural ectodermal markers. Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows), and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Double asterisks indicate lateral displacement owing to widening of the neural plate. See text for details. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
six1
|
|
laevis
|
NF stage 22
|
somite
,
cranial placode
,
anterior placodal area
,
posterior placodal area
|
zic1
|
|
laevis
|
NF stage 22
|
anterior neural fold
,
neural plate border
|
sox11
|
|
laevis
|
NF stage 22
|
head region
,
posterior neural tube
,
anterior neural tube
,
neural tube
,
anterior placodal area
|
sox3
|
|
laevis
|
NF stage 22
|
epibranchial placode
,
cranial placode
,
lateral line placode
,
neural tube
,
anterior placodal area
|
foxd3
|
|
laevis
|
NF stage 22
|
mandibular crest
,
hyoid crest
,
branchial crest
,
posterior neural tube
,
cranial neural crest
|
|
|
Fig. 1 . Induction of ectodermal markers at neural plate boundaries. Ectodermal marker expression in tailbud stage embryos (albino) that had received neural plate grafts (pigmented and/or mycGFP-labeled) into belly ectoderm at stage 13. (A-F′′) Grafts (arrows, outlined) are shown in overviews (A-F) and transverse sections in brightfield (A′-F′), green fluorescent channel (A′-F′), and overlay (A′′-F′′). Sox3 (A-A′′), Sox11 (B-B′′), Zic1 (C-C′′; inset shows higher magnification of expression in graft) and FoxD3 (D-D′′) are maintained or induced (asterisks) in graft. Six1 (F-F′′) is induced in host ectoderm (arrowheads), whereas Sox9 (E-E′′) is induced in the graft (asterisk) and sometimes in host ectoderm (arrowhead). |