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Fig. 7. Expression patterns of neural crest marker and mesodermal genes in Tbx1 knockdown embryos. (A) Snail2, Sox9, and PCNS expressions in the head of stages 22 and 27 embryos as revealed by whole-mount in situ hybridization. Lateral view, anterior to the left. (B) Distribution of the fluorescent neural crest cells in the cranial neural crest-transplantation experiment. The image is flipped horizontally. (C, D) Myf-5, MyoD, and Tbx6 expressions as revealed by whole-mount in situ hybridization. (C) Stage 37, lateral view, anterior to the left. (D) Stage 16, dorsal view, anterior to top. MO-injected sides (*) are shown by Magenta-GAL staining. Images of MO-injected sides are flipped horizontally to facilitate comparison. |