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Fig. 3. In situ expression patterns of xSOX3 (A–D), XProx1 (E–H), and gamma-cry (I–M) mRNA. (A) Left lateral view of the head of a stage-32 embryo. Strong xSOX3 expression is observed throughout the cns and lens. Other areas of expression include what are presumed to be derivatives of the unidentified dorsolateral cranial placodes (black arrow- head). (B–D) Left lateral views of lentectomized larval eyes. (B) Example displaying xSOX3 expression in a 2-day transdifferentiating cornea is seen as a solitary spot centrally located over the pupillary space, indicated by a white arrowhead. (C) Example of a 5-day transdifferentiating cornea that exhibits only background levels of hybridization of the sense-control probe. (D) Example of a younger, 2-day transdifferentiating cornea hybridized with anti-sense probe, which exhibits no expression of xSOX3. (E) Left lateral views of, from left to right, stage 28, 32, and 35 embryos, with the anterior end facing upwards. Expression is first observed broadly within the lens placode, which later becomes restricted to the developing lens. (F–H) Left lateral views of lentectomized larval eyes. (F) Case exhibiting corneal expression in a solitary spot centrally located over the pupillary space after 8 days of transdifferentiation, indicated by a black arrowhead. Naturally occurring dark brown pigment within the pigmented epithelium condenses at the edges of the collapsed pupillary space (white arrowheads). (G) Example of a 6-day transdifferentiating cornea hybrid- ized with sense-control probe demonstrating no detectable hybridization in the cornea. (H) Example of a younger, 2-day transdifferentiating cornea hybridized with anti-sense probe, which exhibits no expression of XProx1. (I) Left lateral view of the head of a stage-30 embryo. Strong expression of 6-cry can clearly be seen in the developing lens. (J–L) Left lateral views of lentectomized larval eyes. (J) Example displaying corneal expression centrally located over the pupillary space in a 7-day transdifferentiating cornea, as indicated by the black arrowhead. (K) Example of a 6-day transdifferentiating cornea exhibiting no hybridization with sense-control probe. (L) Example of a younger, 1-day transdifferentiating cornea hybridized with anti-sense probe, which exhibits no detectable expression of gamma-cry. Partial dotted lines in B–D, F–H, and I–L are included to help demarcate the perimeter of the pupillary space. Abbreviations: cns, central nervous system; lt, lentoid; other abbreviations are as indicated in Figure 2. Scale bar: 200 μm (A, C–D, F–L); 330 μm (B); 800 μm (E). |
