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Fig. 1. Sox21 functions as a repressor to expand neural progenitors and inhibit neuronal differentiation. (A) Diagram of Sox21 proteins used in overexpression studies. (B) In situ hybridization (ISH) of neurula stage embryos injected with sox21 mRNA on right side and analyzed for sox3, ngn2, neuroD or n-tubulin expression. (C) ISH of neurula stage embryos injected with sox21-EnR or sox21-VP16 mRNA on right side and analyzed for sox3, ngn2 or n-tubulin expression. (D) ISH of embryos injected with hormone-inducible GR-sox21 mRNA and analyzed for sox3 or n-tubulin expression. Right: Transverse section of GR-sox21 injected tailbud embryo following ISH for sox3. (E) ISH of embryos injected with chick sox21 mRNA and analyzed for sox3 or n-tubulin expression. |