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Fig. 8. Phenotypes of embryos injected with MO1 or MO2. Control embryos or embryos injected with antisense morpholino oligonucleotides were fixed and subjected to in situ hybridization using the indicated probes. (A–D) Xbra expression at stage 11. Embryos injected with MO1 (C) had reduced levels of Xbra expression, and gastrulation movements were delayed in comparison with control and mMO-injected embryos (A, B). MO2-injected embryos (D) also showed slight retardation of gastrulation. (E–H) Sox3 expression at stage 22. In control and mMO-injected embryos (E, F), Sox3 is expressed in presumptive lens ectoderm (PLE, arrows) and the dorsolateral placode (DLP, arrowheads). In contrast, Sox3 expression is absent or greatly reduced in the PLE and DLP of MO1- or MO2-injected embryos. (I–P) Slug expression at stage 22. Dorsal (I–L) and lateral (M–P) views are shown. Arrowheads (I, J, M, N) or asterisks (K, L, O, P) indicate cephalic neural crests. Note the absence of cephalic neural crest migration and the gap between rows of Xslug expression in the trunk (arrows) in MO1- and MO2-injected embryos. (Q–S) Twist expression at stage 26. Cephalic neural crest migration (arrowheads) is abnormal in MO1 embryos. |
