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Fig. 3. Sox3 inhibition by BMP and sox2 induction by Noggin require de novo protein synthesis. Expression of (A) sox3 and (B) sox2 in ectodermal explants revealed by WISH. Explants were excised at stage 8–9 from untreated embryos or embryos injected with noggin mRNA at the 1-cell stage. Explants were incubated with or without BMP protein and/or cycloheximide (CHX) as indicated at the top of each row. Explants were collected at early gastrula (stage 10.5), late gastrula (stage 12.5) or neurula (stage 17) as indicated to the left. The whole embryo control by which the stage was determined is in the column on the far right. |