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Fig. 3. Xmxi1 is required for primary neurogenesis. (A–L) Whole-mount in situ hybridization of stage 14 embryos injected with Xmxi1-MO (12.5 ng), Xmxi1-MO2 (15 ng), mismatch morpholino MM-Xmxi1-MO (12.5 ng), MT-Xmxi1 (500 pg), or SoxD (200 pg), as indicated in the upper right hand corner. The antisense probes used are indicated in the lower left hand corner. The injected side (β-gal, light blue) is on the right, and embryos are shown as a dorsal view, anterior down with the exception of J and L, which are lateral. (M) Xmxi1 is required for SoxD-induced neuronal differentiation. Animal caps were isolated from embryos injected with SoxD (200 pg), Xmxi1-MO (12.5 ng), Co-MO (12.5 ng), and MT-Xmxi1 (500 pg), as indicated and analyzed by real-time RT-PCR. Expression levels were normalized to ODC and were compared to the induction capacity of SoxD-injected animal caps, which was set to 10. |
