Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
sox2xenopus   

Too many results?Too few results?

Experiment details for sox2

Mamada H et al. (2009) Assay

Involvement of an inner nuclear membrane protein, Nemp1, in Xenopus neural development through an interaction with the chromatin protein BAF.

Gene Clone Species Stages Anatomy
sox2.L laevis NF stage 13 to NF stage 14 neuroectoderm , pre-chordal neural plate , chordal neural plate , neural plate

Display additional annotations [+]
  Fig. 3. Overexpression of Nemp1 causes eye defects and reduces expression of eye marker genes at the neurula stage. Embryos were injected with mRNA for Nemp1 or globin as indicated, together with nβ-gal mRNA. (A) Eye-defect phenotypes at the tailbud stage (stages 40–41). Fractions indicate the proportion of the presented phenotype per total number of informative injected embryos. (B) WISH analysis at the neurula stage (stages 13–14). Examined genes are as indicated. Numbers in red, downregulation; numbers in black, normal expression. ant, anterior view (dorsal is upwards); dor, dorsal view (anterior is upwards); arrowhead, injected side. Amounts of injected mRNAs (pg/embryo): nemp1a, 400; globin, 400.

Gene Clone Species Stages Anatomy
sox2.L laevis NF stage 13 to NF stage 14 neuroectoderm , pre-chordal neural plate , chordal neural plate , neural plate

Display additional annotations [+]
  Fig. 4. Knockdown of Nemp1 by MOab disrupts eye development. (A) MOab inhibits splicing of nemp1 pre-mRNA. (Upper panel) Sequence alignment of exon 1–intron 1 splice junctions. Target sequences of MOa and MOb are underlined. (Lower panel) RT-PCR detection of unspliced transcripts in MOab (25 or 50 ng) or 5mmMO (50 ng)-injected embryos. RT−, RT-PCR without reverse transcriptase reaction. (B) Morphological appearances of injected embryos. MOab (Ba), 5mmMO (Bb), or MOab plus nemp1 mRNA (Bc) were injected together with nβ-gal mRNA as a tracer (red) into the animal pole region of both right blastomeres at the four cell stage. Injected doses (per embryo): MOab 20 ng; nemp1 mRNA, 200 pg. Upper panels, dorsal view (anterior is upwards); lower panels, right side view of the same embryo in the upper panels. (C) WISH analysis of 5mmMO or MOab-injected embryos at stages 13–14. Arrowhead, injected side.