| |
Fig. 3. BMP inhibition together with eFGF activation induces neural marker expression indirectly in Xenopus. (A–L) Inhibition of BMP by injection of Smad6 into the A4 blastomere does not induce either Sox3 (A, B) or Sox2 (C, D) expression. eFGF together with BMP inhibition into the A4 blastomere induces Sox3 (E, F, N) and Sox2 (G, H, O). Neural induction by the former combination is inhibited when Nodal signaling is blocked: injection of Smad6 + eFGF together with CerS no longer induces Sox3 (I, J) or Sox2 (K, L). (M) Quantification of Sox3 and Sox2 expression in the different experiments described above. A, C, E, G, I and K dorsal views. B, D, F, H, J and L ventral views of the embryos to their left. N and O are enlargements of the areas enclosed by a square in F and H, respectively. |
