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Figure 7. Ajuba LIM Proteins Affect Cell Survival and Border Territories without Affecting Proliferation(A) X. laevis embryos were injected with XSlug or XLIMD1 mRNA, fixed at stage 16, and immunohistochemistry performed for phosphohistone-H3.(B) X. laevis embryos were injected with XSlug MO or a combination of XLIMD1 +XWTIP MOs. Embryos were fixed at stage 16 and TUNEL staining performed. The number of embryos displaying increased TUNEL staining on the injected side over the total number of embryos analyzed is shown in the bottom right corner.(C) X. laevis embryos were injected with MOs as shown, fixed at stage 16, and in situ hybridization performed for Epiker (epidermal) and Sox2 (neural). |