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Display additional annotations [+]
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Fig. 2. Sox3 activates geminin and sox2 expression in the absence of protein synthesis. (A) WISH of stage 12 embryos injected with sox3, sox3-VP16, or sox3-EnR mRNA (200–800 pg) at the 1-cell stage. Sox3 and Sox3-VP16 expand expression of sox2 and gem (arrows) and Sox3-EnR induces gem in stage 12 embryos. Images are ventral view with the anterior to the top. (B) High levels of Sox2 and Sox3 induce expression of gem in ectodermal explants. RT-PCR analysis of ectodermal explants for sox2, gem and ef1α expression from embryos injected with noggin (25 pg), sox2, sox3 (500 or 800 pg) and sox3-VP16 (200 pg). Sibling embryos were stage 12. (C) Sox3-GR induces expression of sox2 (Dex, n = 22/30; Dex + CHX, n = 12/20) and gem (Dex, n = 31/35; Dex + CHX, n = 46/54). (D) Sox2-GR induces gem in the absence of protein synthesis (Dex, n = 25/43; Dex + CHX, n = 35/47). WISH of embryos injected with either Sox2-GR or Sox3-GR (400 pg) and incubated without or with Dex (1 μM), CHX (10 μM), or Dex + CHX at either stage 9.5 or 11 and collected at stage 12. Hatched rectangle indicates region of flat mount high magnification shown below whole embryo images. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
zic1
|
|
laevis
|
NF stage 17
|
hyoid crest
,
branchial crest
,
pre-chordal neural plate
,
neural plate
,
anterior neural fold
|
tubb2b
|
|
laevis
|
NF stage 14
|
neuroectoderm
,
chordal neural plate
,
lateral
,
neuron
|
tubb2b
|
|
laevis
|
NF stage 23
|
brain
,
spinal cord
,
neuron
|
neurog1
|
|
laevis
|
NF stage 14
|
neuroectoderm
,
chordal neural plate
,
lateral
,
neuron
|
neurog1
|
|
laevis
|
NF stage 23
|
midbrain-hindbrain boundary
,
spinal cord
|
ncam1
|
|
laevis
|
NF stage 17
|
pre-chordal neural plate
,
anterior neural fold
,
neural tube
|
gmnn
|
|
laevis
|
NF stage 17
|
pre-chordal neural plate
,
neural plate
,
anterior neural fold
|
sox15
|
|
laevis
|
NF stage 17
|
anterior neural fold
,
neural tube
|
|
|
Fig. 1. Sox3 expands the expression of early neural markers and delays the expression of proneural and neuronal markers. (A) WISH of stage 17 neurulae for early neural markers as indicated in lower right corner. (B) WISH of stage 14 neurulae and stage 23 tailbud embryos for the pan-neuronal marker: n-tub and pro-neural marker: neurogenin r-1 ngn. Embryos are injected with sox2 or sox3 (400 or 500 pg) and lacZ (100 pg) mRNA in 1 of 2-cells. Images are a dorsal view with anterior to the bottom and the injected side on the right marked by a black asterisk in the first panel. |