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Fig. 2. eif6 overexpression specifically inhibits eye development. (a)–(j) Embryos were injected with eif6 (400 pg) and βgal (400 pg) or GFP (400 pg) alone into one blastomere of the two-cell stage and harvested at the neurula stage. Whole mount in situ hybridisations were performed for rax (a), (b), pax6 (c), (d), otx2 (e), (f), six3 (g), (h) and sox2 (i), (j), fgf8 (k), (l), en2 (m), (n), egr2 (o), (p).GFP overexpression did not affect the expression of rax (a), pax6 (c), otx2 (e), six3 (g), sox2 (i), fgf8 (k), en2 (m), egr2 (o). Inset in (a) shows diffusion of GFP fluorescence in the whole injected side (i.s.) of a neurula. eif6 overexpression specifically reduced rax (b), pax6 (d), otx2 (f) and six3 (h) expression within the presumptive eye field (arrowheads), while neural expression of the same markers was unaffected (arrows). (j) No expression difference was found for the specific neural markers sox2, fgf8 , en2, egr2 between the eif6-injected and un-injected side. |
