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Fig. 4. Injection of Oct25 mRNA in the ectodermal animal cap (AC) regulates the paraxial marker mesodermal PAPC independent of mesendoderm induction. Animal views (with dorsal up) of embryos from whole mount in situ hybridization, labeled for PAPC, mesoder- mal marker Xbra, endodermal marker Xsox17a, epidermal ectoderm marker XK81A1 (epidermal keratin), neurectodermal marker Sox2. Corresponding values are listed in Table S4. (A) Animal view of an uninjected embryo labeled for PAPC. (B) Frontal section of an unin- jected embryo labeled for PAPC. The insert shows a magnification from the indicated region. (C, D) Animal views of uninjected embryos labeled for Xbra and Xsox17a. (E, F, G, H) Corresponding embryos and section injected with Oct25 mRNA in the animal pole. The insert in (F) shows the ectopic PAPC expression. (I, J) Animal views of uninjected embryos labeled for XK81A1 and Sox2. (K, L) Corresponding embryos injected with Oct25 mRNA in the animal pole. (M) Reverse transcription-polymerase chain reaction (RT-PCR) on uninjected whole embryos (WE), uninjected ACs (AC), Oct25 mRNA injected ACs (Oct25 AC), water control (H2O), one of the corresponding - RT controls. The mesodermal markers PAPC and Xbra, the endodermal marker Xsox17a, the epidermal keratin (XK81), the neurectodermal Sox2, the classical EP/C-cadherin, and H4 were analyzed. (N) Effects of the Oct25 knock down on expression of paraxial protocadherin (PAPC) and Xbra. |
