|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
mycn
|
|
laevis
|
NF stage 41
|
retina
,
ciliary marginal zone
,
retinal neural layer
,
retinal ganglion cell layer
,
retinal inner plexiform layer
|
hes4
|
|
laevis
|
NF stage 41
|
retina
,
ciliary marginal zone
|
hes1
|
|
laevis
|
NF stage 41
|
retina
,
ciliary marginal zone
|
rax
|
|
laevis
|
NF stage 41
|
retina
,
ciliary marginal zone
|
atoh7
|
|
laevis
|
NF stage 41
|
retina
,
ciliary marginal zone
|
ascl1
|
|
laevis
|
NF stage 41
|
retina
,
ciliary marginal zone
|
myc
|
|
laevis
|
NF stage 41
|
retina
,
ciliary marginal zone
|
|
|
Fig. 2. Nutrients are necessary for acquisition of the committed neural progenitor fate but not for the maintenance of the most immature
stem/progenitor cells. (A) Proneural gene expression levels decreased following ND, as did levels of the progenitor markers sox2 and n-Myc (B). Dashed line in B indicates the central domain of the CMZ. n=8, 32 embryos/condition. (C) Expression of the eye field transcription factor gene rx1 remained unchanged. n=4, 16 embryos/condition. (C) c-Myc gene expression levels remained unchanged; however, the domain of c-Myc gene expression was reduced in the central CMZ of ND embryos. n=8, 32 embryos/condition. (D) Expression levels of the stem cell marker genes hes1 and hes2 remained unchanged in ND conditions. n=4, 16 embryos/condition. Scale bars: 50 μm (A,B,D); 50 μm (C; rx1) 25 μm (C; c-Myc). |
|
Display additional annotations [+]
|
|
Fig. 7. Activation of mTOR signalling is sufficient to maintain the progenitor pool. (A,B) Activation of mTOR with bpV rescues pS6 to a level 70% of that of controls in ND retinas (top row), specifically in CMZ progenitor cells (bottom row). Dashed lines encircle the CMZ. n=4, 16 embryos/condition.
(C-F) bpV treatment rescued the downregulation of genes observed following ND, without affecting the expression of stem cell markers. n=4, 16 embryos/ condition. (B-E) Error bars show 95% confidence intervals; ***P≤0.001, *P≤0.05 (unpaired t-test). Scale bars: 100 μm (A, top panel); 50 μm (A, bottom panel); 50 μm (F). |