|
| Gene |
Clone |
Species |
Stages |
Anatomy |
|
sox2.L
|
|
laevis
|
NF stage 14
|
neuroectoderm
,
neural plate
|
|
sox2.L
|
|
laevis
|
NF stage 17
|
neuroectoderm
,
neural plate
|
|
sox2.L
|
|
laevis
|
NF stage 22
|
neuroectoderm
,
neural plate
,
neural fold
,
optic field
|
|
sox2.L
|
|
laevis
|
NF stage 35 and 36
|
otic vesicle
,
lens
,
retina
,
brain
,
forebrain
,
[+]
|
Display additional annotations [+]
|
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Figure 6. Loss of function of SPC7 diminishes expression of neural and eye markers. (A-D) Whole mount hybridization in situ of embryos injected unilaterally with 90 ng SPC7 morpholino. Injected sides are shown to the right. (A) Frontal, dorsal, and lateral views showing Sox2 expression in control (left) and SPC7-MO unilaterally injected (right) embryos. Early- (top row) and mid-neurula (second row) embryos showing diminished Sox2 staining on the SPC7 morpholino injected side, most obviously in the eye anlage but apparent throughout the head folds (arrows). The loss of expression became more obvious at later stages. Row Three: antero-frontal view of stage 22 embryos. Sox2 staining was undetectable in retina, lens, and neural tube, and was reduced in the midbrain on the injected side. Row 4: dorsal view of the same embryos showing that Sox2 staining was absent in the neural tube on the injected side. Bottom row: Stage 35 embryo showed complete loss of Sox2 staining in the eye on the injected side. (B) Lateral views showing Rx2a expression was not detectable on the injected side (arrow). (C) Lateral views showing Pax6 expression pattern in control (left) and injected (right) stage 267 embryos. Pax 6 staining was absent in the eye field on the injected side (arrow). (D) Otx2 expression in uninjected control (left) and SPC7-MO unilaterally injected (right) embryos. Top row: antero-dorsal view of stage 14 embryos showing significantly diminished Otx2 expression on the injected side (arrow). Bottom row: Lateral views of control (left) and SPC7 MO-injected (right) sides of the same embryo showing barely detectable Otx2 expression on the injected side (arrow). (E, F) Frontal sections through the head of stage 35 embryos injected unilaterally (injected side to the right) with 30 ng SPC7-MO showing expression of β-Crystallin (E) and Opsin (F) expression on the non-injected side only. |
