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Fig. 8. Ectodermal explants derived from ventralized embryos are neuralized by Noggin. (A) X. laevis embryos were injected in both blastomeres at the two cell stage with β-catenin MO, then re-injected in the animal pole at the 4 cell stage with 1 or 10 pg of noggin mRNA. At stage 9, animal caps were cut, and cultured to stage 24. Animal caps from β-catenin morphants and control embryos injected with noggin mRNA were analyzed by in situ hybridization for expression of sox2 and nrp1. Animal caps cut from β-catenin noggin injected morphants were equally likely to express sox2 and nrp1 as caps cut from embryos injected with noggin mRNA alone. (B, C) Quantification of the results shown in (A). A repeat of the experiment gave similar results. (D) Animal caps cut from β-catenin morphants injected with noggin mRNA also strongly express both sox2 and definitive neural markers as assayed by non-quantitative RT-PCR. |
