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Fig. 9. Xath5 injection suppresses Sox2 protein levels. (A-F) Control embryos injected with GFP alone and dexamethasone (DEX) treated (A,B), or co-injected with Xath5GR and GFP without DEX (C,D), did not show downregulation of Sox2 protein in the injected areas. By contrast, embryos co-injected with Xath5GR and GFP and DEX treated (E,F) displayed a pronounced Sox2 downregulation in Xath5-expressing cells. (G-H‴) Staining 6 hours after the start of DEX treatment shows a downregulation of Sox2 (G′) and EdU (G′) in areas of the retina that overexpress Xath5GR (G). Areas overexpressing Xath5GR are outlined in a magnified view in H-H‴. Occasionally, a reduction in Sox2 is seen in cells that are still cycling (arrowheads, H‴). |
