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Figure S10. Overexpression or knockdown of PN1 does not affect expression of Gsc,
Xnr3, Vent2, and Sox17a
Whole-mount in situ hybridization of Xenopus embryos at early gastrula stage in vegetal
(A,B,I,J) and lateral view (C-H, K-P). Embryos were injected into the margin (A-H) or animal
pole (K-N) of a single blastomere at the 4-cell stage with nlacZ mRNA as lineage marker (red
nuclei). Embryos in (I,J) were animally and in (O,P) marginally injected. Injected doses per
embryo were: nlacZ mRNA (100 pg), PN1 mRNA (4 ng), MOs (15 ng in K-N; 60 ng in
I,J,O,P). PN1-MO is an equal mixture of PN1.a-MO1, PN1.a-MO2, and PN1.b-MO.
(A,B,I,J) Gsc expression in the dorsal mesoderm.
(C,D,K,L) Xnr3 expression in the dorsal mesoderm.
(E,F,M,N) Vent2 expression in the ventrolateral mesoderm and ventral ectoderm.
(G,H,O,P) Sox17a expression in the endoderm.
Indicated phenotypes were observed in A, 8/8; B, 15/15; C, 7/7; D, 8/8; E, 11/11; F, 14/19; G,
14/14; H, 25/25; I, 11/11; J, 15/15; K, 9/9; L, 10/10; M, 12/12; N, 18/18; O, 30/30; P, 26/26. |
