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Fig. S7. Oct25/60 and Sox2 suppression of mesoderm and endoderm is not blocked by Geminin knockdown. Embryos were injected with the reagents indicated, plus β-galactosidase mRNA, were lineage labeled (Red-gal, pink), and were in situ hybridized to detect Xpo, Brachyury or Sox17 (see Materials and methods). 120 pg of mRNAs (Oct25, Oct60, Sox2, Ezh2), 8 ng of Ezh2MO and Suz12MO, and 1 ng Geminin MO were used. Arrowheads mark injected regions. On the right, embryos were only scored if the lineage label was visible and overlapped expression of the marker; number of embryos analyzed is in parentheses. |
