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Fig. 2. Effects of NotchICD on markers of the three germ layers at gastrula stage. Embryos were injected with 1 ng of notchICD mRNA in one cell at the 4-cell stage and fixed when sibling control embryos reached stage 11. They were revealed by ISH with the following probes: bra (purple staining in A,B,C′,D,E,F′), chd (turquoise staining in A,B,D,E), sox17α (purple staining in G–H′) and sox2 (purple staining in I). Green fluorescence corresponds to immunolocalization of the Myc-tag epitope fused to NotchICD (C,F and insets in G,H,I). (A–C,D–F) Lateral views of notchICD-injected embryos to show changes in the bra domain. The dorsal side (up) is marked by chd staining. Photographs were inverted to facilitate comparisons between the injected- and the non-injected sides. (G,H) Vegetal views of embryos injected with notchICD mRNA to show changes in sox17α expression. (I) Dorsal view of an embryo injected with notchICD mRNA to show changes in sox2 expression. (C′,F′,H′) Sections in the planes shown by the yellow dotted lines in (C,F,H) respectively; animal is up, vegetal is down. Photographs in (C′,F′,G–I) were oriented with the injected side towards the right. nis: non-injected side; is: injected side; spe: subprablastoporal prospective endoderm; bpe: blastoporal prospective endoderm. Arrowheads in (C′) point to the animal border of the bra domain in the pre-involuted mesoderm. Arrows in (G–H′) point to the spe. The dotted red line in (G,I) demarcates the blastopore. |
