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Fig. 5.
Characterization of Sox10-GFP stable transgenic embryos. (A-C) Stage 25 embryos. (A) ISH for endogenous sox10 in wild-type embryos at the indicated stage, side view. (B, C) GFP fluorescence of Sox10-GFP stable transgenic embryos at the indicated stage, B, side view and C, anterior view. (D, E) Stage 30 embryos, side view. (D) ISH for endogenous sox10 in wild type embryos at the indicated stage. (E) GFP fluorescence of Sox10-GFP stable transgenic embryos at the indicated stage. Note the similar expression of Sox10 and GFP in the migrating neural crest cells (arrows). Arrow head indicates the otic vesicle and e the eye. |