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Fig. 4. XAWP1 regulates neural crest induction upstream of Msx1 and
Pax3. (A) Four-cell stage embryos were injected in the animal pole region
with DN BMP4 receptor (1 ng) with or without XAWP1 (0.5, 2 ng), and
the animal cap explants were excised at stage 9 and cultured to stage 11
for RT-PCR analysis. (B-D) Four-cell stage embryos were injected in the
dorso-animal region of one blastomere with AWP1 MO1 (5 ng) with or
without Msx1 (100 pg) or Pax3 (70 pg) as indicated and then subjected to
in situ hybridization against Sox10 at stage 16. |
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Fig. 5. Neural crest induction by FGF or Wnt signal requires XAWP1
function. (A-K) One blastomere of a eight-cell stage embryo was injected
in the dorso-animal region as indicated with FGF8a (20 pg), XWnt8-CSKA
(100 pg), DN XWnt8 (400 pg), XAWP1 (300 pg), pt -catenin (100 pg), VP16-
Tcf3 (50 pg), Co MO (5 ng) and AWP1 MO1 (5 ng) and then subjected to in
situ hybridization against Sox10 at stage 16. Arrows indicate the expanded
expression of Sox10. |
