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Figure 1.
Sox5 Is Required for NC and NPB Formation
(A) In situ hybridization examining FoxD3, Snail2, Sox9, and Sox10 in Xenopus embryos injected with Sox5 MO or Sox5 mRNA. Embryos were collected at midneurula stages (stage 17). Asterisk denotes injected side with β-galactosidase (β-gal) staining (red) as lineage tracer.
(B) Explant assay examining Snail2 and Sox9 in Wnt8/Chordin-induced explants that were injected with Sox5 MO. Explants were collected alongside sibling embryos cultured to midneurula stages (stage 17).
(C) In situ hybridization examining Six1 and Foxi1c in embryos injected with Sox5 MO or Sox5 mRNA. Embryos were collected at early neurula stages (stage 13).
(D) In situ hybridization examining Msx1, Pax3, and Zic1 in embryos injected with Sox5 MO or Sox5 mRNA. Embryos were collected at early neurula stages (stage 13/14).
(E) Explant assay examining Msx1, Pax3, and Zic1 in Wnt8/Chordin-induced explants that were injected with Sox5 MO. Explants were collected alongside sibling embryos cultured until early neurula stages (stage 14). Asterisk denotes injected side with β-gal staining (red) as lineage tracer.
See also Figure S1. |
