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Fig. 5. Xenopus laevis Sox4 and Sox11 regulate neural crest specifier genes FoxD3, Sox10, and Twist. Functional knockdowns of Sox4 and Sox11 lead to the loss of neural crest specifiers on the injected size (shown by lacZ staining) of FoxD3 (A and B), Sox10 (C and D), and Twist (E and F). (I) Quantification of effects on FoxD3 expression: Sox4mo(n=36), Sox10mo(n=33), and Controlmo(n=23) χ2=51.8 with 3 degrees of freedom. (J) Quantification of effects on Sox10 expression: Sox4mo(n=43), Sox11mo(n=20), and Controlmo(n=22). χ2=50.4 with 4 degrees of freedom. (K) Quantification of effects on Twist expression: Sox4mo(n=73), Sox11mo(n=47), and Controlmo(n=24) χ2=90.2 with 4 degrees of freedom. (G–H) SoxCmo injected embryos rescued with SoxC mRNA by FoxD3 expression. (L) Quantification of effects with FoxD3: Sox4mo(n=67), and Sox11mo=(30); (χ2=3.28) with 2 degrees of freedom. |
