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Fig. 2 Spatial expression of Xenopus kctd15 in early development. Whole mount in situ hybridization against kctd15 was performed on embryos from indicated stages. No detectable signal was seen with the sense probe (data not shown). (A-C) At stage 10.5, kctd15 was uniformly expressed throughout the presumptive ectoderm. (D-G) At stage 14, kctd15 expression was detected in the non-neural ectoderm and was excluded from the neural plate. Dorsal is upward in D and E. Anterior is upward in F and G. (H-J) At stage 16, kctd15 expression was detected in the preplacodal ectoderm (ppe), the neural crest (nc) and the roof plate (rf). Dorsal is upward in H and I. Anterior is upward in J. (K-M) At stage 18, kctd15 expression was detected in the neural crest (nc), the roof plate (rf) and the olfactory placode (pOl). Dorsal is upward in K and L. Anterior is upward in M. (N-P) At stage 25, kctd15 expression was detected in the pronephric duct (pd), the mandibular arch (Md), the hyoid arch (Hy), the olfactory placode (pOl), trigeminal ganglia (tg) and the anterodorsal lateral line placode (pAD). Anterior is left. (Q) Whole mount double in situ hybridization against kctd15 and sox10 was performed on stage 17 embryos (middle and right panels). The right panel is an enlarged view of the middle panel. Dark blue and orange dotted lines mark the kctd15-expressing and sox10-expressing area, respectively. For reference, single in situ hybridization against sox10 (red purple) was also performed (the left panel). All panels are dorsal views with anterior to the top. |
