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Fig. 4. The C-terminal domain of Sox10 is sufficient to induce neural crest progenitors. (A) Embryos were injected in one blastomere at the two-cell stage with mRNA encoding either wild type Sox10 (Sox10) or various Sox10 deletion constructs (Delta-Sox10, Sox10-delta-C, or Nsox10) and analyzed for Slug expression at stage 17. Sox10 injection (1 ng) expands Slug expression domain (white arrow). A transverse section through a Sox10-injected embryo illustrates Slug expansion on the injected side (black arrow). Delta-Sox10-injected embryos (1 ng) display a dramatic expansion of the Slug expression domain. By contrast, injection of Sox10-delta-C (2 ng) resulted in a moderate inhibition of Slug expression, whereas NSox10-injected embryos (1 ng) were unaffected. RNA encoding the lineage tracer beta-galactosidase was coinjected to identify the injected side (red staining), the right side in all panels. (B) Schematic representation of wild type Sox10 and various Sox10 deletion constructs. The HMG box is depicted in blue. Quantification of the in situ hybridization results. (1), expansion of Slug expression domain; (2), reduction of Slug expression domain; (3), Slug expression domain is unaffected. N, number of cases analyzed. |
