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Fig. 2. Developmental expression of Sox10 by whole-mount in situ hybridization. (A) Sox10 expression at the neurula stage (stage 14) is detected at the lateral edges of the neural plate. Slug (B) and Sox9 (C) expression at a similar stage are shown for comparison. (A–C) Dorsal views, anterior to top. (D) Transverse section of a stage 14 embryo. Sox10 expression is restricted to the neural crest-forming region. (E–H) Stage 16 embryo, dorsal (E, G) or lateral (F, H) views, anterior to top. As the neural tube closes, Sox10 (E, F) remains strongly expressed in the neural crest at all axial levels. Sox9 expression (G, H) is shown for comparison. (I) At stage 22, Sox10 is detected in migrating cranial neural crest cells (red arrow) as well as in neural crest at the dorsal midline in the trunk region (green arrow). An additional domain of expression includes the otic placode (yellow arrow). Dorsal view, anterior to top. (J–L) In stage 25 embryos, Sox10 is detected in the streams of cranial neural crest that populate the branchial arches (red arrows), in the dorsal aspect of the neural tube (green arrow) and in the otic placode (yellow arrow). Lateral (J) and dorsal (K) views, anterior to left. (L) Transverse section of a stage 25 embryo, highlights Sox10 expression in neural crest progenitors at the dorsal midline. (M-P) At stage 32, Sox10 expression is downregulated in the branchial arches but persists in the otic vesicle (yellow arrow), at the dorsal midline (green arrow), in the forebrain, and in discrete domains adjacent to the hindbrain. Lateral (M) and dorsal (P) views, anterior to left. Higher magnification view of Sox10 expression in the head (N). Sox9 expression in the head of an embryo at a similar stage is shown for comparison (O). In a lateral view of an embryo at stage 35 (Q), Sox10 is detected in the forebrain, the otic vesicle (yellow arrow), a number of cranial ganglia and in differentiating melanoblasts. (R) Transverse section at the level of the hindbrain illustrates Sox10 expression in the otic vesicle (yellow arrow) and adjacent VII/IX cranial ganglion (purple arrow). |
