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Figure S5. Hairy2 and Id3 MO double knockdown leads to a more dramatic reduction of Sox10 expression in NC than individual single knockdown. (A) Embryos unilaterally injected with Hairy2 MOs (10 ng), Id3 MO (15 ng) or both MOs (25 ng total). Note that Sox10 expression is dramatically inhibited in double knockdown (c) (83% totally inhibited, n=24) while residual expression is clearly seen in single knockdown (50% reduced, n=14 for hairy2 MO and 45%, n=17 for Id3 MO). Lateral views of control and injected sides of tailbud embryos are shown. β-galactosidase was used as a lineage tracer.
(B) Q-PCR analysis of NC induced animal cap explants from embryos injected radially with Hairy2 MO (5 ng/blastomere), Id3 MO (10 ng/blastomere) or both (15 ng total/blastomere). Note that both Hairy2 and Id3 MO inhibit Sox10 expression and that the reduction observed is more severe in the double knockdown. |