|
|
| |
|
|
|
| |
Fig. 6. Temporal analysis of transformation of ANF into NC. (A) Representative embryo showing transformation phenotype monitored by the expression of the NC-specific gene Snail2. Note the transformation of the ANF into NC with almost no effect in the medio-lateral axis. (B) Representative embryo showing the expansion phenotype monitored by the expression of Snail2. Note that the expansion of the NC is as much in the anterior–posterior axis as it is in the medio-lateral axis. (C) Diagram to show the experimental design used in panels D and E. Embryos were injected with different doses of β-catenin-GR mRNA at the 32-cell stage and treated with dexamethasone at different stages (green bar), the expression of NC markers was analyzed at stage 16. (D) Analysis of the ANF transformation phenotype and NC expansion phenotype (inset) after injection of 100 pg of β-catenin-GR and activation at different stages. Note that the highest ANF transformation is reached with activation at stage 10–11 while the highest NC expansion phenotype is reached with activation at stage 9. (E) Analysis of the ANF transformation phenotype and NC expansion phenotype (inset) after injection of different doses of β-catenin and activation at stage 11. Note that the higher doses of β-catenin can still induce NC expansion at later stages (inset). |
