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Fig. 1. Xfz7 rather than Xfz3 is expressed in the early neural crest. (A) Embryos were harvested at the stages indicated and expression of Xfz7, Xfz3, Slug and Pax3 was assessed by RT-PCR. Histone H4 was used as a loading control. (B–E) Double label in situ expression of Xfz7 and Xfz3 with Xslug at early neurula (stage 13, B and D) and late neurula stage (stage 18, Ci and Ei). Xfz7 and Xfz3 (red) and Slug (purple). Cii and Eii are frozen sections cut at the level shown in panels Ci and Ei. (F and G) Wholemount in situ expression of panels F slug and G Xfz3 on stage 13 embryos. |
