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Figure 3. Samba is expressed in the neural and neural crest derivatives as seen by in situ hybridization. A: Vegetal view of stage 10 embryos with dorsal side to the right. Samba expression is concentrated in the dorsal region. B: Neurula stage embryo. Samba is present in the neural plate and crest domain. C: Anterior-dorsal view of late neurula stage embryo. Arrows point to the outer edge of the forming migratory crest domain, which expresses Samba. D: Double in situ for Slug and Samba show that Samba overlaps with Slug-expressing migrating streams but is not restricted to neural crest domain. Slug probe was developed with a violet stain and Samba with blue stain. E: Anterior-lateral view of tail bud stage embryo indicates that Samba is expressed in migrating lateral streams of crest cells (arrow) and optic vesicles. F: Lateral view of tail bud stage embryo showing that Samba remains in the crest cells, optic vesicle, and now appears in the otic vesicle as well. G: Dorsal view of a late tail bud stage embryo. H,I: Higher magnification of cranial expression in Figures F and G. |
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Figure 4. Overexpression of Samba does not affect neural and crest markers. Embryos co-injected with 200 pg of Samba and 100 pg of beta-galactosidase in one blastomere at two-cell stage were processed for in situ hybridization for the neural markers Sox2 and Sox3 and the crest markers Slug, Msx1, and Twist. None of the markers altered their expression pattern in the presence of excess Samba. |