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Fig. 6. ADAM19 is critical for neural crest induction. (A) In situ hybridization of neurula (stage 15) embryos using Slug and Sox8 to detect neural crest cells. The injected side is to the left. Embryos injected with MO19 present a decrease expression of both neural crest markers (red arrowhead). (B) Real-time PCR analysis at stage 15. Level of gene expression was measured by the 2(-δCT) method and normalized to α-tubulin. Results are presented as the Log2 of the fold change compared to non injected embryos. Variation of Sox2, Sox8 and Slug are all significant (P < 0.01) in embryos injected with MO19 when compared to the control MO. C) lateral view of tailbud stage embryos (stage 24) visualizing GFP. Embryos were injected at the 16-cell stage in a dorso-lateral-animal blastomere to target neural crest cells. In the control, GFP is found in all CNC segments (white arrows). This is absent in 33% of the embryos injected with MO19. |
