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Fig. 1. Xslug expression and cellular localization of Xslug protein. (A–D) In situ hybridization using a Xslug probe. (A) Vegetal view of a stage 10 embryo. d, dorsal; v, ventral; arrow, dorsal blastopore lip. Note the expression in the marginal zone above the blastopore lip. (B) Stage 10 embryo sectioned sagittally. An, animal pole; Vg, vegetal pole; D, dorsal; V, ventral; arrow, dorsal blastopore lip. Notice that Xslug is expressed in the dorsal marginal zone, including the superficial and deep cells. (C) Dorsal view of a stage 15 embryo showing strong Xslug expression in the neural folds (arrowheads) and weaker expression in the notochord (n). (D) Paraffin section of embryo shown in (C). Arrowheads, neural folds; n, notochord; s, somite. (E) Anti-myc immunostaining of a stage 11 embryo injected with Xslug-myc at the one cell stage. Note the nuclear localization of the Xslug-myc protein (arrows). |
