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Figure 5. Neural crest induction in Xenopus requires Dvl1 and Dvl2, but not Dvl3. A-A rdquo : Unilateral injection of MOs + GFP mRNA targeted to one dorsal lateral blastomere of a 16-cell embryo. Slug expression is lost on the injected side due to Dvl1 and Dvl2 morpholinos. B-B rdquo : Dvl1 and Dvl2 MOs eliminate expression of Twist. C,C': Graphs of combined morpholino data for Slug in situ hybridizations. Data shown are percent of embryos showing normal or abnormal expression of Slug or Twist. Uninjected Control N=16; Dvl-1MO 72 ng, N=26; Dvl-2MO 72 ng, N=10; Dvl-3MO 72 ng, N=12; Dvl-1MO + Dvl-2MNO 36 ng each, N=15; Dvl-1MMMO 72 ng, N=13; Dvl-1MO + Dvl-1MMMO 36 ng each, N=25; Dvl-2MO + Dvl-1MMMO 36 ng each, N=11. D,D': Graphs of combined data for Twist in situ hybridizations. Uninjected Control N=36; Dvl-1MO 72 ng N=84; Dvl-2MO 72 ng, N=28; Dvl-3MO 72 ng, N=20; Dvl-1MO + Dvl-2MNO 36 ng each, N=31; Dvl-1MMMO 72 ng, N=54; Dvl-1MO + Dvl-1MMMO 36ng each, N=18; Dvl-2MO + Dvl-1MMMO 36 ng each, N=24. |