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Fig. 5. Loss of Xfz7 function leads to downregulation of neural crest gene expression in whole embryos and expansion of neural gene expression. Embryos were injected at the 2-cell stage (except K, 8 cell stage) with RNA in the animal pole of one blastomere. MOs or RNA' used were standard control MO (60 ng) (A, D, G, J, M, P), Xfz7MO (60 ng except (K) 40 ng) (B, E, H, N, Q) and Xfz7CRD (1 ng) (C, F, I, L, O, R). All injections contained the lineage tracer β-galactosidase (250 pg). Thus, the red nuclear β-galactosidase staining indicates the injected side. The dark purple indicates the expression of FoxD3 (A–C), Sox10 (D–F), Sox2 (G–I), Sox9 (J–L), Snail (M–O) and Twist (P–R). All embryos are dorsal views with anterior to the top. The dashed line in panels H and I indicates the embryonic midline. |
