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Figure 4. Ajuba LIM Protein Expression in Xenopus Embryos Enhances Neural Crest Development in a Slug-Dependent Manner(A) X. laevis embryos coinjected with β-gal and either XSlug, XSnail, mAjuba, mLIMD1, mWTIP, XLIMD1, or XWTIP capped mRNAs were fixed at stage 18 and in situ hybridization performed for XSlug.(B) Graph displaying the percent of embryos with increased neural crest on the injected side (by Slug in situ hybridization). The total number of embryos injected is shown over each column (n).(C) X. laevis embryos were coinjected with β-gal and the XSlug MO alone or in combination with XLIMD1, XWTIP, or XSlug mRNA, and in situ hybridization for Twist performed.(D) Graph displaying the percent of embryos with decreased neural crest on the injected side (by Twist in situ hybridization). The total number of embryos injected is shown over each column (n). |
