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Figure 6. Effects of FoxN3 depletion on neural crest formation. Embryos were unilaterally injected with 13 ng FoxN3-MO and 500 pg GFP RNA. After sorting for right/left injections, embryos were submitted at different developmental stages to in situ whole mount hybridization. The asterisks indicate the side of injection. A: FoxD3, stage 23. B:AP2 alpha , stage 23. C:snail, stage 23. D:AP2 alpha , stage 29. E:snail, stage 29. No difference in the expression domains between injected and uninjected sides can be observed. F,G: Whole mount in situ hybridization of unilaterally injected embryos stained for HoxA2 (F) and Sox3 (G). Embryos were injected at their right sides. H-K: Whole mount in situ hybridization of bilaterally FoxN3-MO or Co-MO-injected embryos (13 ng pro blastomere). H:Xbap, Co-MO-injected, stage 38. I:Xbap, FoxN3-MO injected, stage 38. Xbap expression is visible close to the cement gland in the controls (see arrow). The FoxN3-MO-injected embryos show reduced expression of Xbap in the prospective jaw (see arrow). The ventral part of this expression domain is completely absent. J:Xbap, Co-MO-injected, stage 43, ventral view. K:Xbap, FoxN3-MO-injected, stage 43, ventral view. The Xbap expression in the controls is restricted at stage 43 within the head to Meckel's cartilage. FoxN3-MO-injected embryos show severe reduction of staining (see arrows). |
