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Figure 2. Loss of p2ry4 affected neural crest and placode development. Uninjected (a, d, g, j), TALEN‐L injected (b, e, h, k), and TALEN‐L/R injected (c, f, i, l) embryos were subjected to WISH for snail1 (a–f) and hairy2b (g–l). Snail1 expression at early neurula (a–c) and early tailbud (d–f) stages. Snail1 expression was reduced in TALEN‐L/R injected embryos (c, f, red arrowheads). hairy2b expression at early (g–i) and middle (j–l) neurula stages. hairy2b expression at the anterior neural plate border was reduced in TALEN‐L/R injected embryos. Anterior (a–c, j–l), lateral (d–f), and dorsal (g–i) views. ot, otic vesicle; nc, neural crest; fp, floor plate. Red arrowheads indicate migrating neural crest cells. Brackets indicate prospective floor plate |
