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Figure 7. Whole-mount ISH for well-known markers of craniofacial development in embryos injected with mRNA encoding D71V.
Shown is a subset of the WISHs performed (see also Supplementary Fig. S3). This set represents at least two markers each of the relevant tissues, i.e. neural crest and the three placodes studied. A, chart showing locations of marker expression. B, normal (1st column), over-expressed (2nd column) and under-expressed (3rd column) examples from each of five representative markers. Red arrows point to positions of abnormal signal patterns; the green arrow in v points to normal expression on the side opposite the disrupted pattern. The patterns we saw, even in injected embryos that did not have significantly more misexpression than background, are consistent with incorrect or incomplete differentiation (ii, iii, v, vi, viii, ix) and anomalies in neural crest migration (xi, xii, xiv, xv). To date, we have not detected any correlation between the types or magnitudes of disruptions caused and the identity of the ion flux-perturbing construct injected. |
